Zymobiomics DNA Miniprep Kit
| Cat# | Name | Size |
|---|---|---|
| D4300 | Zymobiomics DNA Miniprep Kit | 50 Preps |
Documents
Protocol:
Data Sheet:
SDS (MSDS):
Optimized Lysis Protocols:
Description
Highlights
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Description
Applicable For
All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume
≥ 50 µl DNA Elution Buffer or DNase free water
Equipment
Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume
Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×109 bacterial, 2×108 yeast, or 2×107 mammalian cells)
Purity
High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample Source
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
Sample Storage
Eluted DNA should be stored at ≤ -20°C.
Size Range
Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type
Total DNA
Yield
Up to 25 µg total DNA can be eluted into ≥50 µl
| Cat# | Name | Size |
|---|---|---|
| C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack |
| S6012-50 ZR | BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes |
| C1078-50 | Zymo-Spin IICR Columns | 50 Pack |
| D4300-1-40 | ZymoBIOMICS Lysis Solution | 40 ml |
| D4300-2-100 | ZymoBIOMICS DNA Binding Buffer | 100 ml |
| D4300-3-50 | ZymoBIOMICS DNA Wash Buffer 1 | 50 ml |
| D4300-7-30 | ZymoBIOMICS HRC Prep Solution | 30 ml |
| D4300-4-60 | ZymoBIOMICS DNA Wash Buffer 2 | 60 ml |
| D4302-5-1 | ZymoBIOMICS DNase/RNase Free Water | 1 ml |
| D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml |
| C1057-50 | Zymo-Spin III-F Filters | 50 Pack |
