ZymoBIOMICS RNA Miniprep Kit
| Cat# | Name | Size |
|---|---|---|
| R2001 | ZymoBIOMICS RNA Miniprep Kit | 50 Preps |
| R2002 | ZymoBIOMICS DNA/RNA Miniprep Kit | 50 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Optimized Lysis Protocols:
Description
Highlights
- Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
- Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms.
Description
The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
Equipment
Microcentrifuge, vortex, cell disrupter (recommended)
Purity
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.
Size Range
Total RNA ≥17 nt
Yield
100 µg RNA (binding capacity), ≥50 µl (elution volume)
 |
98 / 100 Bioz Stars |
Metagenome sequencing-based strain-level and functional characterization…
J Oral Microbial – Published 28 Dec 2018
Sterile forceps (a new pair for every sample) were used to remove the points from the tubes.. Each plaque sample was pelleted by centrifugation at maximum speed, washed twice in 1 ml PBS, and digested by re-suspending in 250 μl PBS containing 25 μl Metapolyzme (Sigma, USA), and incubating at 37° for 3 h. The lysate was used for DNA extraction employing the ZymoBiomics miniprep kit (Zymo Research, Germany) according to the manufacturer’s instructions.. Quantity and quality of the extracts were assessed using a Qubit 3.0 (ThermoFisher Scientific, USA).
Genotype-Environment Interaction Shapes the Microbial Assemblage in Grap…
Microorganims – Published 21 Sep 2018
DNA was extracted from each sample by using the ZymoBiomics DNA MicroPrep Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instructions.. To access bacterial communities, the V4 region of the 16S ribosomal gene was amplified using primers 515F and 806R and fungal community diversity
Assembly of 913 microbial genomes from metagenomic sequencing of the cow…
Nat Commun – Published 28 Feb 2018
riefly, the sample was spun down for 2 min at 6000 rpm, rinsed with PBS and spun down again (5 min at 6000 rpm), prior to removing the supernatant.. Cells were lysed with glass bead disruption in a detergent buffer, and DNA was extracted using the ZymoBiomics DNA Mini Kit (Zymo Research).. For shotgun library creation, 100 ng was sheared to ~400 bp average insert length and used to create a library using the HyperPrep kit (KAPA Biosystems).
| Cat # | Name | Size |
|---|---|---|
| R1100-50 | DNA/RNA Shield | 50 ml |
| W1001-30 | DNase/RNase-Free Water | 30 ml |
| D7001-1-50 | DNA/RNA Lysis Buffer | 50 ml |
| D7010-2-50 | DNA/RNA Prep Buffer | 50 ml |
| D7010-3-24 | DNA/RNA Wash Buffer (Concentrate) | 24 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1006-50-F | Spin-Away Filters | 50 Pack |
| C1006-50-G | Zymo-Spin IIICG Columns | 50 Pack |
| C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack |
| E1010-1-4 | DNA Digestion Buffer | 4 mL |
- Catalog#: R2001
- Package Length (in Inches): 9
- Package Width (in Inches): 65
- Package Height (in Inches): 6
- Package Weight (in Pounds): 2.6
- Size: 50 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
