ZymoBIOMICS Spike-in Control II (Low Microbial Load)
| Cat# | Name | Size |
|---|---|---|
| R2135 | ZymoBIOMICS MagBead DNA/RNA | 1 x 96 Preps |
| R2136 | ZymoBIOMICS MagBead DNA/RNA | 4 x 96 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
Highlights
- Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc
- Ultra-Pure: High-quality DNA/RNA (including mall/microRNAs) are inhibitor-free and ready for RT/qPCR and microbiome measurements using Next-Gen sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms
Description
The ZymoBIOMICS MagBead DNA/RNA kit provides a high-throughput, magnetic bead-based purification of both high-quality DNA and total RNA (including small/microRNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water. DNA/RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR,, etc.
Binding Capacity
15 µg DNA/RNA per 30 µl ZymoBIOMICS MagBinding Beads.
Equipment Needed
Magnetic stand or separator, heat block, liquid handler or robotic sample processer (user provided).
Purity
High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc
Recommended Materials
(sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).
Sample Preservation DNA/RNA Shield
lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures
Sample Types
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50 mg of mammalian feces, ≤ 50 mg soil, and 5-20 mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs
Size Limits
Capable of recovering DNA and total RNA ≥ 17 nucleotides.
Storage
DNA and RNA eluted with ZymoBIOMICS DNase/RNase-Free Water (provided)can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.
| Cat# | Name | Size |
|---|---|---|
| R1100-50 | DNA/RNA Shield | 50 ml |
| R1100-250 | DNA/RNA Shield | 250 ml |
| R1101 | DNA/RNA Shield-Fecal Collection Tube | 10 pack |
| R1103 | DNA/RNA Shield Lysis Tubes (Microbe) | 50 pack |
| R1104 | DNA/RNA Shield Lysis Tubes w/Swab (Microbe) | 50 pack |
| R1106 | DNA/RNA Shield Collection Tube w/Swab (1 ml fill) | 10 pack |
| R1107 | DNA/RNA Shield Collection Tube w/Swab (1 ml fill) | 50 pack |
| D3001-2-20 | Proteinase K w/ Storage Buffer Set | 20 mg |
| D3001-2-5 | Proteinase K w/ Storage Buffer Set | 5 mg |
| D4302-5-1 | ZymoBIOMICS DNase/RNase Free Water | 1 ml |
| D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml |
| D4302-5-30 | ZymoBIOMICS DNase/RNase Free Water | 30 ml |
| D4302-5-50 | ZymoBIOMICS DNase/RNase Free Water | 50 ml |
| D4302-6-12 | ZymoBIOMICS MagBinding Beads | 12 ml |
| D4302-6-6 | ZymoBIOMICS MagBinding Beads | 6 ml |
| D7001-1-200 | DNA/RNA Lysis Buffer | 200 ml |
| D7001-1-50 | DNA/RNA Lysis Buffer | 50 ml |
| D7010-2-200 | DNA/RNA Prep Buffer | 200 ml |
| D7010-2-50 | DNA/RNA Prep Buffer | 50 ml |
| E1010 | DNase I Set | 250 U |
| R2130-1-120 | MagBead DNA/RNA Wash 1 | 120 ml |
| R2130-1-30 | MagBead DNA/RNA Wash 1 | 30 ml |
| R2130-2-20 | MagBead DNA/RNA Wash 2 | 20 ml |
| R2130-2-80 | MagBead DNA/RNA Wash 2 | 80 ml |
| S6002-96-3 | ZR-96 BashingBead Lysis Rack | 0.5 mm & 0.1 mm |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes |
| R1102 | DNA/RNA Shield Collection Tube | 50 pack |
- Catalog#: R2135
- Package Length (in Inches): 8
- Package Width (in Inches): 6.5
- Package Height (in Inches): 6
- Package Weight (in Pounds): 2
- Size: 1 x 96 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
