ZymoBIOMICS Spike-in Control II (Low Microbial Load)
Cat# | Name | Size |
---|---|---|
D6321 | ZymoBIOMICS Spike-in Control II (Low Microbial Load) | 25 Preps |
D6321-10 | ZymoBIOMICS Spike-in Control II (Low Microbial Load) | 250 Preps |
Description
Highlights
- Absolute Quantification: Enables cell number measurements from low bacterial load samples using Next-Gen Sequencing.
- In situ Quality Control: Ensures each sample is quantified accurately.
- Log Abundance Distribution: Three microbes alien to the human microbiome in log-distribution from 103 to 105 cells.
Description
ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. They represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.
16S rRNA genes
https://s3.amazonaws.com/zymo-files/BioPool/D6321.refseq.zip
Biosafety
This product is not biohazardous as microbes have been fully inactivated.
Impurity Level
< 0.01% foreign microbial DNA
Sample Storage
-80°C
Storage Solution
DNA/RNA Shield™
- Catalog#: D6321 / D6321-10
- Package Length (in Inches): 5.3
- Package Width (in Inches): 3.3
- Package Height (in Inches): 0.5
- Package Weight (in Pounds): 0.1
- Size: 25 Preps /250 Preps
- Unit Standard: Metric
- Volume Units: Milliliters