Zymoclean Gel RNA Recovery Kit
| Cat # | Name | Size |
|---|---|---|
| R1011 | Zymoclean Gel RNA Recovery Kit | 50 Preps |
Documents
Protocol:
SDS (MSDS):
Description
Highlights
- Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- High Recovery: ≥ 80% recovery for RNA > 500 nt.
Description
The Zymoclean Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).
Equipment
Microcentrifuge, 37°C-65°C heat source
Purity
RNA is ready for all subsequent analysis and molecular manipulation
Sample Source
Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.)
Size Range
RNA fragments ≥500 nt (≥80% recovery)
Yield
10 µg RNA (binding capacity), ≥6 µl (elution volume)
Q1: Are the columns and wash buffer interchangeable with the RCC-5 Kit?
Yes, the Zymo-Spin IC Columns and RNA Wash Buffer are interchangeable.
Q2: Can the Zymoclean be used for samples in solution (without running on a gel)?
No, it’s not optimized for samples in solution. High efficiency recovery is not guaranteed and the success of the purification can be variable.
Q3: What is the maximum weight of gel slice I can use?
Up to 400 mg of agarose.
Q4: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Q5: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
Q1: Are the columns and wash buffer interchangeable with the RCC-5 Kit?
Yes, the Zymo-Spin IC Columns and RNA Wash Buffer are interchangeable.
Q2: Can the Zymoclean be used for samples in solution (without running on a gel)?
No, it’s not optimized for samples in solution. High efficiency recovery is not guaranteed and the success of the purification can be variable.
Q3: What is the maximum weight of gel slice I can use?
Up to 400 mg of agarose.
Q4: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Q5: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
Dimeric RNA was extracted from gel slice using the Zymoclean Gel RNA Recovery kit for Northern analysis of signal recognition particle (SRP) RNA.
Rulli Jr SJ, et al. Selective and Nonselective Packaging of Cellular RNAs in Retrovirus Particles. Journal of Virology. 2007.
The Zymoclean Gel RNA Recovery kit was used on agarose gels with RNA derived from Candida albicans strains. Researchers showed new mechanisms that regulates rRNA processing involving 18S, 5.8S and 25S rRNAs.
<Pendrak, ML et al. Ribosomal RNA processing in Candida albicans. RNA Journal. 2011.
A dsRNA full-length transcript standard was visualized by electrophoresis on a 1% denaturing agarose gel and isolated using the Zymoclean Gel RNA Recovery kit. This rotavirus (RVA) dsRNA was used in qRT-PCR for RVA detection with the limit of detection being 1 genome copy per reaction.
Mijatovic-Rustempasic S et al. Sensitive and Specific Quantitative Detection of Rotavirus A by One-Step Real-Time Reverse Transcription-PCR Assay without Antecedent Double-Stranded-RNA Denaturation. Journal of Clinical Microbiology. 2013.
