Zymoprep-96 Yeast Plasmid Miniprep

Zymoprep-96 Yeast Plasmid Miniprep

Cat#NameSize
D2005Zymoprep-96 Yeast Plasmid Miniprep2 x 96 Preps
D2006Zymoprep-96 Yeast Plasmid Miniprep4 x 96 Preps
D2007Zymoprep-96 Yeast Plasmid Miniprep8 x 96 Preps

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Description

  • Simple: Quickly and easily rescue plasmid from yeast.
  • Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
  • High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.

Description

The Zymoprep-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase added in the first solution. There is no need for glass beads or phenol. The Zymoprep-96 incorporates our Zymoprep II kit into a 96-well format resulting in a high-throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.

Technical Specifications

Applicable For

Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications

Elution Volume

≥ 10 µl per well

Equipment

Centrifuge with microplate carriers

Processing Volume

≤ 1.5 ml of Culture

Sample Source

Cell Culture (Colonies/Patches or Liquid Culture)

Size Range

Up to 25 kb.

Yield

Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures

Product FAQ

Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?

Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.

Q2: What is the typical plasmid yield?

Typically, between 0.01 – 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.

Q3: Can this kit be used to isolate linear plasmid DNA?

Yes

Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?

We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.

Q5: What yeast strains are these kits compatible with?

Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis

Citation

99 / 100
Bioz Stars

Tracking the origin of two genetic components associated with transposab…

Nat Common – Published 7 Feb 2019

Plasmids were isolated from individual yeast clones using the Zymo Yeast Plasmid Miniprep kit (Zymo Research) and transformed into Escherichia coli for plasmid purification and sequence

Reprogramming a Deubiquitinase into a Transamidase

ACS Chem Biol – Published 03 Jan 2019

n subsequent rounds, 5 × 106 cells were harvested and reacted with 5, 0.5, and 0.1 μ M of biotinylated Ub D77.. The reaction time was 10 min for all selection rounds except round 5, which was 5 min. At the end of rounds three and five, plasmids were isolated from the enriched clones using a Zymoprep yeast plasmid miniprep kit (Zymo Research) and electroporated into DH10 β cells.. Individual colonies were grown, miniprepped, and sequenced using p C T ¯ y u h l ¯ r 2 to identify beneficial mutations for autoubiquitination.

A user-friendly platform for yeast two-hybrid library screening using ne…

PLoS One – Published 21 Dec 2018

The cDNA inserts of the prey plasmids (pDEST22-insert) were PCR-amplified using backbone-specific primers ( ) and Sanger-sequenced.. Colonies of the Y2H screening plates were dissolved and pooled in 10–15 mL of ultrapure water and plasmids were collected using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research, Irvine, CA, USA).

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Kit Components

Cat # Name Size
D2004-1-45 Solution 1 Digestion Buffer 45 ml
D2004-1-90 Solution 1 Digestion Buffer 90 ml
D2004-2-45 Solution 2 Lysis Buffer 45 ml
D2004-2-90 Solution 2 Lysis Buffer 90 ml
D2004-3-180 Solution 3 Neutralizing Buffer 180 ml
D2004-3-90 Solution 3 Neutralizing Buffer 90 ml
D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml
D4003-2-48 DNA Wash Buffer (Concentrate) 48 ml
C1001-50 Collection Tubes 50 Pack
C2002 Collection Plate 2 Plates
C2003 Elution Plate 2 Plates
C2004 Zymo-Spin I-96 Plate 2 Plates
P1001-2 96-Well Block 2 Blocks
C2007-8 96-Well Plate Cover Foil 8 Foils
C2011-2 Air Permeable Sealing Cover 2 Pack
C2011-4 Air Permeable Sealing Cover 4 Pack
C2011-8 Air Permeable Sealing Cover 8 Pack

Additional Information

  • Catalog#: D2005 /D2006 /D2007
  • Package Length (in Inches): 13
  • Package Width (in Inches): 8
  • Package Height (in Inches): 6.5
  • Package Weight (in Pounds): 5.25 /16.1 /0.1
  • Size: 2 x 96 Preps /4 x 96 Preps /8 x 96 Preps
  • Unit Standard: Metric
  • Volume Units: Milliliters

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