ZymoPURE II Plasmid Midiprep Kit
| Cat# | Name | Size |
|---|---|---|
| D4200 | ZymoPURE II Plasmid Midiprep Kit | 25 Preps. |
| D4201 | ZymoPURE II Plasmid Midiprep Kit | 50 Preps |
Documents
Protocol:
Datasheet"
SDS (MSDS):
Description
Highlights
- Fastest: Simple 20 minute Midipreps.
- Highest Yield: Purify up to 400 µg of plasmid DNA directly from a spin-column.
- Ultra-Pure: EndoZero, vaccine grade, and transfection ready.
Description
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Applicable For
Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.
Binding Capacity
400 µg
Culture Input
≤ 50 ml
Elution Volume
≥ 100 µl
Endotoxin Levels
≤ 0.025 EU/µg Plasmid DNA
Equipment
Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge
Format
Spin Column
Processing Time
20 min
Purity
Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Size Range
Up to 200 kb
Yield
≤ 400 µg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)
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96 / 100 Bioz Stars |
Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Mal…
Nature Communications – Published 16 Feb 2019
Vector constructs were prepared using ZymoPURE plasmid midiprep kit (CatD4201, Zymo Research, CA, USA) following manufacturer’s instructions.
Molecular safeguarding of CRISPR gene drive experiments
Plasmids used for injection into eggs were purified with ZymoPure Midiprep kit (Zymo Research).
Improving cytidine and adenine base editors by expression optimization a…
Nat Biotechnol – Published 29 Nov 2018
All plasmids were assembled by either the USER cloning method as previously described or by Gibson assembly .. Plasmids for mammalian cell transfections were prepared using an endotoxin removal plasmid purification system, ZymoPURE Plasmid Midiprep (Zymo Research Corporation).. HEK293T cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Corning) supplemented with 10% fetal bovine serum (FBS),
“It went beyond my expectation. The protocol is very easy to follow. Very good DNA yields and the DNA was high quality”
– J. G., Yale University
“This kit it pretty great, midiprep DNA in about the time it takes to do a miniprep.”
– T. W., Northeastern University
“My student had an easy time with this kit also, and he had never done a DNA prep before.”
– K. G., Friedrich-Alexander University Erlangen-Nuremberg
- Catalog#: D4200 /D4201
- Package Length (in Inches): 13 /12
- Package Width (in Inches): 8.5 /10.5
- Package Height (in Inches): .13
- Package Weight (in Pounds): 9.3 /7.6
- Size: 25 Preps /50 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
