ZymoPURE Plasmid Miniprep Kit
| Cat# | Name | Size |
|---|---|---|
| D4212 | ZymoPURE Plasmid Miniprep Kit | 800 Preps |
| D4211 | ZymoPURE Plasmid Miniprep Kit | 400 Preps |
| D4210 | ZymoPURE Plasmid Miniprep Kit | 100 Preps |
| D4208T | ZymoPURE Plasmid Miniprep Kit | 10 Preps |
| D4209 | ZymoPURE Plasmid Miniprep Kit | 50 Preps |
Documents
Protocol:
SDS (MSDS):
Description
Highlights
- Highest Yield: Purify up to 100 µg of plasmid DNA in as little as 25 µl directly from a spin-column.
- Transfection-Grade: 50,000 times fewer endotoxins than industry leading minipreps.
- BAC/YAC/PAC Ready: Purify DNA up to ~200 kb.
Description
Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Applicable For
Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.
Binding Capacity
100 mg
Culture Input
≤ 5 L
Elution Volume
≥ 25 ml
Endotoxin Levels
≤ 1 EU/µg
Equipment
Microcentrifuge and/or vacuum manifold
Format
Spin Column
Processing Time
50 min
Purity
Typical Abs 260/280 ≥1.8 and Abs 260/230 ≥ 2.0
Recommended Sample Volume
≤ 5.0 ml
Size Range
Up to ~200 kb
Yield
Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.
Q1: What is the composition of the ZymoPURE™ Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q2: I ran out of ZymoPURE™ Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?
No, the ZymoPURE™ kits are only compatible with ZymoPURE™ Wash 2 and we cannot disclose a substitution due to the sensitive nature of the recipe. Additional ZymoPURE™ Wash 2 can be purchased separately.
Q3: What type of vacuum pump do you recommend?
The vacuum pump should be a single or double-staged unit capable of producing at least 400 mm Hg pressure at the vacuum manifold. If less pressure is applied, centrifuge the column prior to washing to remove any residual lysate/buffer remaining in the matrix.
Q4: Are the ZymoPURE kits compatible with any commercially available vacuum manifold?
Yes, any vacuum that uses standard luer-lock connectors is compatible.
Q5: Can an in-house vacuum line be used with the EZ Vac Vacuum Manifold?
Yes, however, the pressure needs to be around 400 mm Hg. Users should take caution, as the pressure of an in-house vacuum line can fluctuate drastically or be significantly reduced, depending on the demand in the building.
Q6: Can the ZymoPURE Kits be used with other bacteria?
Yes, please contact technical support for an application note.
Q7: Is there a protocol for low-copy number plasmid DNA?
Yes, we do have an application note for low-copy number plasmid available. Please contact technical support for a copy.
Q8: Can I process more than the recommended volume of bacterial culture or use enriched growth media?
Yes, however, more attention is required during the lysis and steps. We recommend centrifuging/pelleting the neutralized lysate before loading onto the supplied syringe/bottle-top filter. For high copy plasmids using more culture can cause overloading and subsequent clogging of columns, which can result in poor DNA yield and/or quality.
Q9: Can the ZymoPURE kits be used to isolate large plasmid constructs (BAC/PAC)?
Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. To increase elution of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ºC) and increasing the incubation time on column up to 10 minutes prior to centrifugation.
 |
96 / 100 Bioz Stars |
Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Mal…
Nature Communications – Published 16 Feb 2019
Vector constructs were prepared using ZymoPURE plasmid midiprep kit (CatD4201, Zymo Research, CA, USA) following manufacturer’s instructions.
Molecular safeguarding of CRISPR gene drive experiments
Plasmids used for injection into eggs were purified with ZymoPure Midiprep kit (Zymo Research).
Improving cytidine and adenine base editors by expression optimization a…
Nat Biotechnol – Published 29 Nov 2018
All plasmids were assembled by either the USER cloning method as previously described or by Gibson assembly .. Plasmids for mammalian cell transfections were prepared using an endotoxin removal plasmid purification system, ZymoPURE Plasmid Midiprep (Zymo Research Corporation).. HEK293T cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Corning) supplemented with 10% fetal bovine serum (FBS),
| Cat # | Name | Size |
|---|---|---|
| D4200-1-100 | ZymoPURE P1 (Red) | 100 ml |
| D4200-1-13 | ZymoPURE P1 (Red) | 13 ml |
| D4200-1-210 | ZymoPURE P1 (Red) | 210 ml |
| D4200-1-3 | ZymoPURE P1 (Red) | 3 ml |
| D4200-2-100 | ZymoPURE P2 (Green) | 100 ml |
| D4200-2-13 | ZymoPURE P2 (Green) | 13 ml |
| D4200-2-210 | ZymoPURE P2 (Green) | 210 ml |
| D4200-2-3 | ZymoPURE P2 (Green) | 3 ml |
| D4200-3-100 | ZymoPURE P3 (Yellow) | 100 ml |
| D4200-3-13 | ZymoPURE P3 (Yellow) | 13 ml |
| D4200-3-210 | ZymoPURE P3 (Yellow) | 210 ml |
| D4200-3-3 | ZymoPURE P3 (Yellow) | 3 ml |
| D4200-4-110 | ZymoPURE Binding Buffer | 110 ml |
| D4200-4-14 | ZymoPURE Binding Buffer | 14 ml |
| D4200-4-3 | ZymoPURE Binding Buffer | 3 ml |
| D4200-5-20 | ZymoPURE Wash 1 | 20 ml |
| D4200-5-320 | ZymoPURE Wash 1 | 320 ml |
| D4200-6-23 | ZymoPURE Wash 2 (Concentrate) | 23 ml |
| D4200-6-28 | ZymoPURE Wash 2 (Concentrate) | 28 ml |
| D4200-7-12 | ZymoPURE Elution Buffer | 12 ml |
| D4200-7-30 | ZymoPURE Elution Buffer | 30 ml |
| D4200-7-6 | ZymoPURE Elution Buffer | 6 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1055-50 | Zymo-Spin II-P Columns | 50 Pack |
- Catalog#: D4212 /D4211 /D4210 /D4208T /D4209
- Package Length (in Inches): 12 /12 /9 /2.5 /7
- Package Width (in Inches): 10.5 /10.5 / 6.5 /2.2 /5
- Package Height (in Inches): 16 /8 /6 /1 /4.5
- Package Weight (in Pounds): 10.6 /5 /2 /0.8 /0.8
- Size: 800 Preps /400 Preps /100 Preps /10 Preps /50 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
