Zyppy Plasmid Miniprep Kit
| Cat# | Name | Size |
|---|---|---|
| D4019 | Zyppy Plasmid Miniprep Kit | 100 Preps |
| D4020 | Zyppy Plasmid Miniprep Kit | 400 Preps |
| D4036 | Zyppy Plasmid Miniprep Kit | 50 Preps |
| D4037 | Zyppy Plasmid Miniprep Kit | 800 Preps |
Documents
Protocol:
SDS (MSDS):
Description
Highlights
- Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
- CPellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
Description
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
Applicable For
Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume
≥ 30 µl
Equipment
Microcentrifuge and/or a vacuum manifold.
Processing Time
8 min
Purity
Typical Abs260/280 ≥1.8.
Size Range
Up to 25 kb
Yield
Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
 |
99 / 100 Bioz Stars |
Expansion of the fatty acyl reductase gene family shaped pheromone commu…
The resulting vectors containing FAR sequences N -terminally fused with 6×His tag were subsequently transformed into E. coli DH5α cells (Invitrogen). . The plasmids were isolated from bacteria with Zyppy Plasmid Miniprep kit (Zymo Research) and Sanger sequenced prior to transformation into yeast.. The protein-coding sequences of all studied FARs were deposited to GenBank (see Key Resources Table for accession numbers).
Engineered microbial biofuel production and recovery under supercritical…
Nat Commun – Published 4 Feb 2019
10 min to serve as template for the PCR reaction using OneTaq Quick-Load Master Mix (NEB) and appropriate sequencing primers (Supplementary Table ). . Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis.. Standard Sanger sequencing of isolated plasmids was used to verify identity (Genewiz).
Discovery of Next-Generation Antimicrobials through Bacterial Self-Scree…
Cells were harvested after 2, 3, and 4 hours for the defined peptide library and harvest after 4 hours for the random peptide library.. Plasmids were isolated from each culture using the Zyppy Plasmid Miniprep kit from Zymo Research Corp. (Irvine, CA).. Plasmid concentrations were measured using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE).
“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min. Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”
– Rebecca N.
“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard). The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”
– Richard F.
“The results gave what they promised! Clean DNA”
– Pamela R (UT Medicine San Antonio)/p>
| Cat # | Name | Size |
|---|---|---|
| D4036-1-12 | 7X Lysis Buffer (Blue) | 12 ml |
| D4036-1-48 | 7X Lysis Buffer (Blue) | 48 ml |
| D4036-1-6 | 7X Lysis Buffer (Blue) | 6 ml |
| D4036-2-160 | Neutralization Buffer (Yellow) | 160 ml |
| D4036-2-20 | Neutralization Buffer (Yellow) | 20 ml |
| D4036-2-40 | Neutralization Buffer (Yellow) | 40 ml |
| D4036-3-120 | Endo-Wash Buffer | 120 ml |
| D4036-3-15 | Endo-Wash Buffer | 15 ml |
| D4036-3-30 | Endo-Wash Buffer | 30 ml |
| D4036-4-12 | Zyppy Wash Buffer (Concentrate) | 12 ml |
| D4036-4-48 | Zyppy Wash Buffer (Concentrate) | 48 ml |
| D4036-4-6 | Zyppy Wash Buffer (Concentrate) | 6 ml |
| D4036-5-20 | Zyppy Elution Buffer | 20 ml |
| D4036-5-5 | Zyppy Elution Buffer | 5 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1019-50 | Zymo-Spin IIN Columns | 50 Pack |
- Catalog#: D4019 /D4020 /D4036 /D4037
- Package Length (in Inches): 7 /13 /7 /12
- Package Width (in Inches): 5 /8 /5 /10.5
- Package Height (in Inches): .4.5 /6.5 /4.5 /8
- Package Weight (in Pounds): 1.5 /3.5 /0.8 /6.2
- Size: 100 Preps /400 Preps /50 Preps /800 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
