Standard Hybridization Reagent Kit

The Twist Standard Hybridization Reagent Kit v2 is the gold standard for efficient and specific binding of panel probes to your regions of interest. Coupled with Twist’s Catalog or Custom Panels, this standard hybridization workflow provides the optimal conditions for a broad range of content.


KEY BENEFITS

Optimum Coverage
  • Delivers excellent uniformity and increased on-target rates
  • Fully automatable
  • Cost-efficient, even coverage
Accommodates a Broad Range of Panels
  • Standardized conditions require no optimization
  • Compatible with fixed content, custom, or spike-ins

Optimized Out of the Box

Coverage obtained with Twist Core Exome (top panels) and coverage with the Twist Core Exome + exon spike-ins. Both conditions provided optimal coverage without changes to the hybridization conditions using the standard Hybridization and Wash Kit.

Maximize sequencing efficiency with even coverage

Even coverage throughout the target results in the most cost-efficient sequencing, and can increase sequencing throughput with higher multiplexing capabilities. Or use this extra sequencing flow cell space to get greater depth on the same number of samples.

Standard Hybridization v2 SKUs

104445: Twist Standard Hyb and Wash Kit v2, 2 Reactions
104446: Twist Standard Hyb and Wash Kit v2, 12 Reactions
104447: Twist Standard Hyb and Wash Kit v2, 96 Reactions
104133: Twist Standard Wash Buffers v2, 2 reactions
104135: Twist Standard Wash Buffers v2, 12 reactions
104137: Twist Standard Wash Buffers v2, 96 reactions


Standard Hybridization v1 SKUs

101279: Twist Hybridization and Wash Kit, 2 Reactions
104178: Twist Hybridization and Wash Kit with Amplification Mix, 12 Reactions
101025: Twist Hybridization and Wash Kit, 12 Reactions
104179: Twist Hybridization and Wash Kit with Amplification Mix, 96 Reactions
101026: Twist Hybridization and Wash Kit, 96 Reactions