Clonal Genes
Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high-quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.
Benefits
- From 9¢ per bp
- No hidden sub-cloning or DNA complexity fees
- From 10 business days
- 0.3 – 5 kb genes cloned into a plasmid of your choice
- Choose a Twist Catalog Vector or send us yours
- 4 prep scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg)
- Normalization and endotoxin free options available
- No order limits
- Same turnaround regardless of order size
Sequence Perfect Genes
A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
Clonal Genes Success Rate
Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.
Gene Fragments
Synthetic Gene Fragments are an inexpensive, rapid, and efficient way to build the genes you need for your research. Gene Fragments from Twist Bioscience improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.
Accurate fragment synthesis accelerates discovery. Twist Bioscience Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.
Benefits
- From 7¢ per bp
- From 6 business days
- Gene Fragments for assembly and cloning
- Compatible with all downstream cloning methods
- Industry leading error rate
- 100 ng – 1 µg yield
- 300 bp – 1800 bp gene fragments
- No order limit
- Fixed price per base pair regardless of length
Best In Class Performance
Twist’s DNA synthesis technology exhibits extremely low error rates. This level of precision makes larger gene assembly more reliable and easier to achieve.In a direct comparison with competitor products Twist outperformed with the lowest error rate. The same sequences were ordered from Twist and Integrated DNA Technologies, Inc. (IDT) for genes of various lengths, the genes were then cloned and transformed, colonies were picked and sequenced. The data represent the average error rate across the given sequences in each set.As seen in the figure below, Twist Gene Fragments had a nearly 2-fold improvement in error rate over IDT gBlocks across the entire size range and a 1.7-fold improvement over eBlocks across the range of 300 – 800 bp, with a 1:5,367 bp and 1:6,253 bp error rate respectively. Low error rates translate into fewer colonies that need to be picked and screened to find your perfect gene saving you time and money.
Affordable Gene Synthesis
Accurate fragment synthesis accelerates discovery. Twist Bioscience Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones. Twist’s lower overall error rate results in more perfect sequences. The performance and scale of Twists Gene synthesis translate into a lower price and less effort per gene. The following figure illustrates the cost per gene ordered as a Gene Fragment from Twist Bioscience. Buying ready-to-use genes is easier, faster, and less expensive than you thought.
