Zymobiomics DNA Miniprep Kit

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Zymobiomics DNA Miniprep Kit

Cat#NameSize
D4300Zymobiomics DNA Miniprep Kit 50 Preps

Documents

Protocol:

Data Sheet:

SDS (MSDS):

Optimized Lysis Protocols:

Categories: , ,

Description

Highlights

  • Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
  • Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
  • Certified Low Bioburden: Boost your detection limit for low abundance microbes.

Description

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

Technical Specifications

Applicable For

All sensitive downstream applications such as qPCR and Next-Generation Sequencing.

Elution Volume

≥ 50 µl DNA Elution Buffer or DNase free water

Equipment

Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).

Processing Volume

Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×109 bacterial, 2×108 yeast, or 2×107 mammalian cells)

Purity

High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8

Sample Source

Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.

Sample Storage

Eluted DNA should be stored at ≤ -20°C.

Size Range

Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.

Type

Total DNA

Yield

Up to 25 µg total DNA can be eluted into ≥50 µl

Kit Components

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