ZymoBIOMICS Spike-in Control II (Low Microbial Load)

ZymoBIOMICS Spike-in Control II (Low Microbial Load)

Cat#NameSize
D6321ZymoBIOMICS Spike-in Control II (Low Microbial Load)25 Preps
D6321-10ZymoBIOMICS Spike-in Control II (Low Microbial Load)250 Preps

Documents

Protocol:

SDS (MSDS):

Description

Highlights

  •  Absolute Quantification: Enables cell number measurements from low bacterial load samples using Next-Gen Sequencing.
  •  In situ Quality Control: Ensures each sample is quantified accurately.
  •  Log Abundance Distribution: Three microbes alien to the human microbiome in log-distribution from 103 to 105 cells.

Description

ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrixImtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. They represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.

16S rRNA genes

https://s3.amazonaws.com/zymo-files/BioPool/D6321.refseq.zip

Biosafety

This product is not biohazardous as microbes have been fully inactivated.

Impurity Level

< 0.01% foreign microbial DNA

Sample Storage

-80°C

Storage Solution

DNA/RNA Shield™

  • Catalog#: D6321 / D6321-10
  • Package Length (in Inches): 5.3
  • Package Width (in Inches): 3.3
  • Package Height (in Inches): 0.5
  • Package Weight (in Pounds): 0.1
  • Size: 25 Preps /250 Preps
  • Unit Standard: Metric
  • Volume Units: Milliliters